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Question 1:

What unique macromolecule is predominantly found in bacteria?





Topic: Biochemical Composition of Bacteria

Correct Answer: C) Peptidoglycan

Detailed Hints:
  • Bacteria are known to possess a unique macromolecule, which is instrumental in maintaining their structural integrity.
  • Among the options provided, "Peptidoglycan" is the correct answer. It is essentially a sugar-protein complex, also known as Murein, which forms a major component of the bacterial cell wall.
  • Therefore, the correct answer is C, "Peptidoglycan".

Question 2:

Which among the following shares a similarity with mesosomes found in bacteria?





Topic: Cell Biology - Structure and Function

Correct Answer: A) Mitochondria in eukaryotes

Detailed Hints:
  • Mesosomes in bacteria are invaginations of the plasma membrane that play a role in cellular processes like respiration.
  • In the options provided, mitochondria in eukaryotes are the closest in function, as they are the powerhouses of the cell, involved in energy production.
  • Hence, the correct answer is A, "Mitochondria in eukaryotes".

Question 3:

How would you classify Clostridium tetanus in terms of its shape?





Topic: Microbiology - Bacteria Classification

Correct Answer: A) Rod-shaped

Detailed Hints:
  • Bacteria are identified and classified based on their morphological characteristics, one of which is shape.
  • Clostridium tetanus, the bacterium responsible for tetanus, is rod-shaped.
  • Therefore, the correct answer is A, "Rod-shaped".

Question 4:

Which type of bacteria is characterized by a thick peptidoglycan layer?





Topic: Microbiology - Bacterial Cell Wall

Correct Answer: A) Gram-positive bacteria

Detailed Hints:
  • The composition of the bacterial cell wall, particularly the thickness of the peptidoglycan layer, is a crucial factor in differentiating types of bacteria.
  • Gram-positive bacteria are characterized by a thick peptidoglycan layer, whereas gram-negative bacteria have a thin peptidoglycan layer and an additional lipopolysaccharide layer.
  • As such, the correct answer is A, "Gram-positive bacteria".

Question 5:

Which of the following is not a component of a flagellum?





Topic: Microbiology - Bacterial Cell Structure

Correct Answer: D) Fimbriae

Detailed Hints:
  • A flagellum is a tail-like structure found in many bacteria, and it is integral to their mobility.
  • It typically consists of a basal apparatus, a short, curved hook, and a helical filament.
  • On the contrary, fimbriae, or pilus, is a completely different structure used for adhesion. Hence, it's not a component of a flagellum.
  • So, the correct answer is D, "Fimbriae".

Question 6:

At 100°C or more, how long does it take to kill vegetative forms of bacterial pathogens?





Topic: Microbiology - Effect of Heat on Bacteria

Correct Answer: D) 10 minutes

Detailed Hints:
  • Extreme heat is often used as a method of sterilization to kill bacteria and other pathogens.
  • Typically, it takes around 10 minutes at 100°C or more to kill vegetative forms of bacterial pathogens.
  • Thus, the correct answer is D, "10 minutes".

Question 7:

What is the term for the location where bacterial chromosomes concentrate?





Topic: Microbiology - Bacterial DNA Structure

Correct Answer: C) Nucleoid

Detailed Hints:
  • In bacteria, the genetic material is not enclosed within a nuclear membrane.
  • Instead, it is found in a part of the cell called the nucleoid, where the bacterial chromosome concentrates.
  • Therefore, the correct answer is C, "Nucleoid".

Question 8:

Which of the following can resist cysts but not spores?





Topic: Microbiology - Spore and Cyst resistance

Correct Answer: D) Heat

Detailed Hints:
  • Both cysts and spores are forms that bacteria can take to survive in unfavorable conditions.
  • While both can survive desiccation and exposure to light, only spores can resist extreme heat. Cysts tend to be more sensitive to high temperatures.
  • Hence, the correct answer is D, "Heat".

Question 9:

Which bacteria type does not form colonies?





Topic: Microbiology - Bacterial Colony Formation

Correct Answer: B) Spirilla

Detailed Hints:
  • Bacterial colonies are clusters of bacteria that originate from a single bacterium.
  • Among the options provided, Spirilla, which are corkscrew-shaped bacteria, do not form clusters or colonies.
  • Therefore, the correct answer is B, "Spirilla".

Question 10:

What type of bacteria is responsible for causing leprosy?





Topic: Microbiology - Bacterial Diseases

Correct Answer: A) Gram-positive

Detailed Hints:
  • Leprosy is a chronic infectious disease caused by Mycobacterium leprae, a type of bacteria.
  • Mycobacterium leprae is a Gram-positive bacterium.
  • Thus, the correct answer is A, "Gram-positive".

Question 11:

Which type of bacteria does not have flagella?





Topic: Microbiology - Bacterial Motility

Correct Answer: B) Cocci

Detailed Hints:
  • Flagella are tail-like structures that many bacteria use for movement.
  • Cocci, which are spherical bacteria, generally do not have flagella and hence are non-motile.
  • Therefore, the correct answer is B, "Cocci".

Question 12:

What term is used when the entire surface of a bacteria is covered with flagella?





Topic: Microbiology - Bacterial Flagella Distribution

Correct Answer: C) Peritrichious

Detailed Hints:
  • The distribution of flagella on a bacterial cell can take several forms.
  • When the entire surface of a bacterial cell is covered with flagella, the term used is "Peritrichious".
  • Thus, the correct answer is C, "Peritrichious".

Question 13:

What is another name for peptidoglycan?





Topic: Microbiology - Bacterial Cell Wall Components

Correct Answer: A) Murein

Detailed Hints:
  • Peptidoglycan, a crucial component of the bacterial cell wall, is a complex sugar-protein molecule.
  • Another term for peptidoglycan is "Murein".
  • Therefore, the correct answer is A, "Murein".

Question 14:

Which type of bacteria has a concentration of flagella on one end only?





Topic: Microbiology - Bacterial Flagella Arrangement

Correct Answer: B) Lophotricus

Detailed Hints:
  • Flagella arrangement varies among bacteria and can often be used as a characteristic for identification.
  • Bacteria that have a tuft of flagella at one end are referred to as "Lophotricus".
  • Consequently, the correct answer is B, "Lophotricus".

Question 15:

Which technique leads to the oxidation of chemical components in bacterial cells?





Topic: Microbiology - Sterilization Techniques

Correct Answer: B) Dry heat

Detailed Hints:
  • Sterilization techniques are designed to eliminate all microbial life, including bacterial cells.
  • Dry heat sterilization works through the process of oxidation, effectively "burning" the chemical components of bacterial cells.
  • Therefore, the correct answer is B, "Dry heat".

MCQs 16:

In which organism is the peptidoglycan found in the cell wall?





Topic: Microbiology - Cell Wall Composition

Correct Answer: A) Bacteria

Detailed Hints:
  • The presence of peptidoglycan is a defining characteristic of certain types of cellular life.
  • Specifically, peptidoglycan is a key component of the cell walls of bacteria, but not of Archaea.
  • As such, the correct answer is A, "Bacteria".

MCQs 17:

Which sterilization method involves removing water from contaminated material to inhibit bacterial growth and reproduction?





Topic: Microbiology - Sterilization Techniques

Correct Answer: C) Desiccation

Detailed Hints:
  • Various sterilization techniques are used to control bacterial growth.
  • Desiccation is a method that involves the extraction of water from contaminated material. Without water, bacteria are unable to grow or reproduce.
  • Therefore, the correct answer is C, "Desiccation".

MCQs 18:

Which of the following is a Gram-negative bacterium?





Topic: Microbiology - Bacterial Classification

Correct Answer: A) Neisseria gonorrhea

Detailed Hints:
  • Bacteria are classified into Gram-positive and Gram-negative based on their reaction to Gram staining.
  • Neisseria gonorrhea is a Gram-negative bacterium, while Clostridium is not.
  • Thus, the correct answer is A, "Neisseria gonorrhea".

MCQs 19:

Which organism listed is considered a prokaryote?





Topic: Microbiology - Cell Type Classification

Correct Answer: C) Both

Detailed Hints:
  • Organisms can be classified as either prokaryotic or eukaryotic based on their cellular structure.
  • Both Bacteria and Archaea are prokaryotes, meaning they lack a nucleus and other membrane-bound organelles.
  • Hence, the correct answer is C, "Both".

MCQs 20:

Which substance eradicates all bacteria and their endospores, although may require 4-8 hours of exposure?





Topic: Microbiology - Sterilization Techniques

Correct Answer: A) Ethylene oxide

Detailed Hints:
  • Sterilization is the process of eliminating or killing all forms of microbial life, including endospores.
  • Ethylene oxide is a gas sterilant known for its ability to kill all microbes and their endospores, although it may require an exposure time of 4-8 hours.
  • Thus, the correct answer is A, "Ethylene oxide".

MCQs 21:

Identify the bacterium that is classified as a bacillus.





Topic: Microbiology - Bacterial Shapes and Arrangements

Correct Answer: C) Clostridium tetani

Detailed Hints:
  • Bacteria are classified based on their shape and arrangement.
  • Bacillus refers to rod-shaped bacteria, and "Clostridium tetani" falls into this category.
  • Therefore, the correct answer is C, "Clostridium tetani".

Question 22:

Which of the following does not retain the Gram stain?





Topic: Microbiology - Gram Staining

Correct Answer: B) Gram-negative

Short Hint:
  • Gram staining is a technique used to differentiate bacterial species into two large groups: Gram-positive and Gram-negative.
  • Gram-negative bacteria do not retain the crystal violet stain used in Gram staining due to their unique cell wall structure.
  • Consequently, the correct answer is B, "Gram-negative".

Question 23:

How many hours of exposure are needed for Ethylene oxide to effectively kill microbes and endospores?





Topic: Microbiology - Sterilization Techniques

Correct Answer: A) 4 - 8

Short Hint:
  • The efficiency of sterilants in eradicating microbes and endospores can be influenced by the duration of exposure.
  • Ethylene oxide, a potent sterilant, requires a specific amount of time to fully eliminate microbial life.
  • The correct exposure time for Ethylene oxide is between 4 to 8 hours, making the correct answer A, "4 - 8".

Question 24:

What is the causative agent of pneumonia?





Topic: Microbiology - Bacterial Diseases

Correct Answer: A) Gram-positive bacteria

Short Hint:
  • Pneumonia, a respiratory disease, can be triggered by different types of organisms.
  • In many cases, Gram-positive bacteria are the causative agents of pneumonia.
  • As such, the correct answer is A, "Gram-positive bacteria".

Question 25:

Which substance is Glutaraldehyde less irritating but more effective than?





Topic: Microbiology - Disinfectants

Correct Answer: C) Formalin

Short Hint:
  • Disinfectants vary in their irritability and effectiveness against microbes.
  • Glutaraldehyde is a disinfectant that is less irritating but more effective than some other substances.
  • In comparison to Formalin, Glutaraldehyde is less irritating but more potent, making the correct answer C, "Formalin".

Question 26:

Which system is found in Photoautotrophic bacteria?





Topic: Microbiology - Bacterial Photosynthesis

Correct Answer: A) Photosystem I

Short Hint:
  • Photoautotrophic bacteria are capable of carrying out photosynthesis, a process remarkably similar to the one in green plants.
  • Bacteria typically utilize Photosystem I and a type of chlorophyll known as bacterial chlorophyll "a" for this process.
  • Therefore, the correct answer is A, "Photosystem I".

Question 27:

Which substance is commonly used to preserve biological specimens?





Topic: Microbiology - Biological Specimen Preservation

Correct Answer: A) Formaldehyde

Short Hint:
  • Preservation of biological specimens is of paramount importance in microbiology to maintain their original morphology and characteristics during study.
  • Among the listed substances, Formaldehyde, also known as formalin, is widely used for this purpose.
  • Hence, the correct answer is A, "Formaldehyde".